一、产品简介：

谷胱甘肽通常以还原型状态(GSH) 存在，但是GSH在氧化应激的作用下会转化为氧化型状态 (GSSG)。因此GSH/GSSG的比值被认为是氧化应激研究的一个重要指标。

本试剂盒含有GSH掩蔽剂，加入掩蔽剂可以除去样品溶液中的GSH，并在谷胱甘肽还原酶作用下使氧化型谷胱甘肽（GSSG）转化为还原型谷胱甘肽（GSH），进而与DTNB与反应生成在412nm处有特征吸收峰的复合物；进而对GSSG进行定量。

二、所需的仪器和用品：

酶标仪、96孔板、低温离心机、水浴锅、可调节移液器、蒸馏水。

1. Min Zhou. 2021. γ-Aminobutyric Acid (GABA) Priming Improves Seed Germination and Seedling Stress Tolerance Associated With Enhanced Antioxidant Metabolism, DREB Expression, and Dehydrin Accumulation in White Clover Under Water Stress. Frontiers in Plant Science. IF=5.57

2. 刘梅，李祖然，张光群，王吉秀，祖艳群. 2020. 硫素对小花南芥体内含硫化合物与铅累积特征的影响.农业环境科学学报.

3.Min Zhou.2021.γ-Aminobutyric Acid (GABA) Priming Improves Seed Germination and Seedling Stress Tolerance Associated With Enhanced Antioxidant Metabolism, DREB Expression, and Dehydrin Accumulation in White Clover Under Water Stress.IF=5.754

4.Ying Zhou.2022.Proteomic Investigation of Molecular Mechanisms in Response to PEG-Induced Drought Stress in Soybean Roots.IF=4.658

5.Congcong Wang.2019.Characterization of the cellular effects and mechanism of arsenic trioxide-induced hepatotoxicity in broiler chickens.IF=3.067

6.Yue Liu.2022.Decreased thioredoxin reductase 3 expression promotes nickel-induced damage to cardiac tissue via activating oxidative stress-induced apoptosis and inflammation.IF=4.109

7.Ma, Wenxue.2022.The role of selenoprotein M in nickel-induced pyroptosis in mice spleen tissue via oxidative stress.IF=5.19

8.Shufeng Wang.2023.Differentially-expressed genes related to glutathione metabolism and heavy metal transport reveals an adaptive, genotype-specific mechanism to Hg2+ exposure in rice (Oryza sativa L.).IF=9.988

9.Erting Fu.2023.Chitosan Reduces Damages of Strawberry Seedlings under High-Temperature and High-Light Stress.IF=3.949

10.Xingang Li.2023.GmGSTU23 Encoding a Tau Class Glutathione S-Transferase Protein Enhances the Salt Tolerance of Soybean (Glycine max L.).IF=6.208

11.Rong Wu.2023.GsNAC2 gene enhances saline-alkali stress tolerance by promoting plant growth and regulating glutathione metabolism in Sorghum bicolor.IF=3

1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？

     答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。

          “24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。

2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？

     答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;

           微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。

3、问：分光法试剂盒与微板法试剂盒是否能通用？

     答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！