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谷胱甘肽过氧化物酶(GPX)试剂盒-可见显色法

货号:G0204W
规格:96样
价格:710
说明书:点击下载
提示:官网说明书如与试剂盒内纸质版有出入,请以纸质版为准!

  • 产品简介
  • 已发文章
  • 产品问答


一、产品简介:

谷胱甘肽过氧化物酶(GPx,EC 1.11.1.9)代表一种具有过氧化物酶活性的酶家族,在保护生物免受氧 化损伤中起重要作用。以往以过氧化氢为底物进行检测,则会受到分解过氧化氢的过氧化氢酶(Catalase)的干扰,本试剂盒提供的有机过氧化物试剂(Cum-OOH)不会被过氧化氢酶分解,因而可以特异地检测出谷胱甘肽过氧化物酶的活力。 因此本试剂盒可以定量检测总谷胱甘肽过氧化物酶。GSH 和 DTNB 反应产生黄色物质,后者在 412nm 下有最大吸收峰,而 GSH-Px 催化有机过氧化物试剂(Cum-OOH)氧化 GSH,使 GSH 量减少,GSH 量减少越多,反应混合液黄色越浅,则 GSH-Px 活性越大;反之,黄色越深,GSH-Px 活性越低。


二、所需的仪器和用品:

酶标仪、96孔酶标板、低温离心机、移液器、研钵。

1. 刘梅,李祖然,张光群,王吉秀,祖艳群. 2020. 硫素对小花南芥体内含硫化合物与铅累积特征的影响.农业环境科学学报.


2. 牟凤利,杨京民,刘翠,刘才鑫,刘梅,陈建军,祖艳群,王吉秀.2021.野生小花南芥体内 AsA-GSH 循环对土壤 CdPb 胁迫的响应.农业环境科学学报.


3.Yue Wang.2021.Exogenous melatonin alleviates NO2 damage in tobacco leaves by promoting antioxidant defense, modulating redox homeostasis, and signal transduction.IF=10.588


4.Rui Jia.2022.Effects of Stocking Density on the Growth Performance, Physiological Parameters, Redox Status and Lipid Metabolism of Micropterus salmoides in Integrated Rice–Fish Farming Systems.IF=7.675


5.Sihong Liu.2022.Combined effects of S-metolachlor and benoxacor on embryo development in zebrafish (Danio rerio).IF=7.129


6.Sihong Liu.2020.Developmental toxicity and transcriptome analysis of zebrafish (Danio rerio) embryos following exposure to chiral herbicide safener benoxacor.IF=6.551


7.Yang Wang.2021.Protective Effect of Lactobacillus plantarum P8 on Growth Performance, Intestinal Health, and Microbiota in Eimeria-Infected Broilers.IF=5.64


8.Fuqi Wang.2021.Isorhamnetin, the xanthine oxidase inhibitor from Sophora japonica, ameliorates uric acid levels and renal function in hyperuricemic mice.IF=5.396


9.Cheng Wang.2022.Hepatoprotective effect of phillygenin on carbon tetrachloride-induced liver fibrosis and its effects on short chain fatty acid and bile acid metabolism.IF=4.36


10.Chen, Siting.2022.Overexpression of the intertidal seagrass J protein ZjDjB1 enhances tolerance to chilling injury.IF=2.496


11.Chen, Siting.2022.Overexpression of the intertidal seagrass 14-3-3 gene ZjGRF1 enhances the tolerance of transgenic Arabidopsis to salt and osmotic stress.IF=2.496


12.Dong  Yin.2022.Effects of stocking density on the growth performance, mitophagy, endocytosis and metabolism of Cherax quadricarinatus in integrated rice–crayfish farming systems.IF=4.755


13.Hongbo Zhang.2022.Trx CDSP32-overexpressing tobacco plants improves cadmium tolerance by modulating antioxidant mechanism..IF=5.437


14.Mengqi Zhang.2023.Effects of light on growth, feeding rate, digestion, and antioxidation in juvenile razor clams Sinonovacula constricta.IF=5.135


15.Zhaojun Chen.2023.Protective effects of Fagopyrum dibotrys on oxidized oil-induced oxidative stress, intestinal barrier impairment, and altered cecal microbiota in broiler chickens.IF=4.014


16.Quanjun Zhan.2023.Effect of copper sulphate on Cryptocaryon irritans based on metabolome analysis.IF=2.58


17.Qianmin Lin.2023.Effect of Sodium Hyaluronate on Antioxidant and Anti-Ageing Activities in Caenorhabditis elegans.IF=5.561


18.Weixu Diao.2023.Effects of Stocking Density on the Growth Performance, Physiological Parameters, Antioxidant Status and Lipid Metabolism of Pelteobagrus fulvidraco in the Integrated Rice-Fish Farming System..IF=3.231


19.Rong Wu.2023.GsNAC2 gene enhances saline-alkali stress tolerance by promoting plant growth and regulating glutathione metabolism in Sorghum bicolor.IF=3


1、问:官网上试剂盒规格标注的“24“48“96是什么意思呢?

     答:“24“48“96是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。

          “24“48“96规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。

 

2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"微板法是什么区别?

     答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;

           微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。

 

3、问:分光法试剂盒与微板法试剂盒是否能通用?

     答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!