一、产品简介：
土壤过氧化氢酶主要分解土壤中的过氧化氢，降低土壤中过度累积的过氧化氢对植 物根系的危害。本试剂盒提供一种简单，灵敏，快速的测定方法，即 CAT 催化过氧化 氢产生水与氧气，剩余的过氧化氢与一种高灵敏显色探针反应生成有色物质，其在 510nm 左右有最大吸收峰。通过过氧化氢的减少量来计算土壤中 CAT 酶的活力。 本试剂盒突出特点是从紫外波长（240nm：过氧化氢的检测波长）转换到可见波长 （510nm）检测，无需使用石英比色皿或 UV 板。而且由于过氧化氢极其不稳定，直接 检测造成读值不稳定，且蛋白质等组分在此紫外波长下也有光吸收，影响结果精确性。
二、所需的仪器和用品：
可见分光光度计、1mL 玻璃比色皿（光径 1cm）、台式离心机、水浴锅、可调式移液器
1.Bin Zhou. 2020. Spatial distribution of phthalate esters and the associated response of enzyme activities and microbial community composition in typical plasticshed vegetable soils in China. Ecotoxicology and Environmental Safety. IF=6.3
2.J.Y. Zheng. 2022. Forty-year-old orchards promote carbon storage by changing aggregate-associated enzyme activities and microbial communities. Catena. IF=5.20
3.Yinjie Zhang. 2021. Long-term organic substitution management affects soil phosphorus speciation and reduces leaching in greenhouse vegetable production. Journal of Cleaner Production. IF=9.3
4.ZHANG Yin-Jie. 2021. Effects of a decade of organic fertilizer substitution on vegetable yield and soil phosphorus pools, phosphatase activities, and the microbial community in a greenhouse vegetable production system. Journal of Integrative Agriculture. IF=2.8
5.Zaid Khan.2022.How Biochar Affects Nitrogen Assimilation and Dynamics by Interacting Soil and Plant Enzymatic Activities: Quantitative Assessment of 2 Years Potted Study in a Rapeseed-Soil System..IF=.6.627
6.Kai Cai.2021.Pyrolysis-assisted transesterification for accurate quantification of phospholipid fatty acids: Application to microbial community analysis in 1000-years paddy soil chronosequence.IF=6.114
7.Ruiting Wang.2022.Metabolomics and Microbiomics Reveal Impacts of Rhizosphere Metabolites on Alfalfa Continuous Cropping.IF=6.064
8.Yan Wang.2022.Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng.IF=6.064
9.Bin Zhou.2020.Spatial distribution of phthalate esters and the associated response of enzyme activities and microbial community composition in typical plastic-shed vegetable soils in China.IF=4.872
1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
