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内切-β-1,4-葡聚糖酶/纤维素酶试剂盒

货号:G0533F
规格:48样
价格:560
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  • 产品简介
  • 已发文章
  • 产品问答

一、产品简介:

内切-β-1,4-葡聚糖酶(EC3.2.1.4)存在于细菌、真菌和动物体内,是纤维素酶系的组份之一,这类酶随机水解β-1,4-糖苷键,将无定形长链纤维素分子截短,将其分解成葡萄糖、纤维二糖、纤维三糖等各种类型的还原糖,在碱性条件下,产生的还原糖能与3,5-二硝基水杨酸反应生成棕红色物质,该物质在540nm下有最大吸收峰,即可得出内切-β-1,4-葡聚糖酶的酶活性大小。


二、所需的仪器和用品:

可见分光光度计、1mL玻璃比色皿(光径1cm)、水浴锅、离心机、可调式移液器、研钵、冰和蒸馏水。


1.《Inhibitory effects and mechanisms of vanillin on gray mold and black rot of  cherry tomatoes》.2021.《Pesticide Biochemistry and Physiology》.IF=3.9


2.《Rumen Fermentation, Digestive Enzyme Activity, and Bacteria Composition between Pre-Weaning and Post-Weaning Dairy Calves》.2021.《Animals》.IF=2.75


3.《Effects of Age, Diet CP, NDF, EE, and Starch on the Rumen Bacteria Community and Function in Dairy Cattle》.2021.《Microorganisms》.IF=3.94


4.Chengze Yu.2021.Hydrothermal pretreatment contributes to accelerate maturity during the composting of lignocellulosic solid wastes.IF=9.624


5.Yi Gao.2022.Full-Scale of a Compost Process Using Swine Manure, Human Feces, and Rice Straw as Feedstock..IF=6.064


6.Dao-Jun Guo.2021.Differential Protein Expression Analysis of Two Sugarcane Varieties in Response to Diazotrophic Plant Growth-Promoting Endophyte Enterobacter roggenkampii ED5.IF=5.754


7.Yangyi Hao.2021.Rumen Fermentation, Digestive Enzyme Activity, and Bacteria Composition between Pre-Weaning and Post-Weaning Dairy Calves.IF=2.752


8.Fanlin Kong.2022.Preliminary Investigation of the Effects of Rosemary Extract Supplementation on Milk Production and Rumen Fermentation in High-Producing Dairy Cows.IF=7.675


9.Yujie Song.2022.Enzymes and microorganisms jointly promote the fermentation of rapeseed cake.IF=6.59


10.Yu-Xuan Wu.2022.Inhibitory effect and mechanism of action of juniper essential oil on gray mold in cherry tomatoes.IF=6.064


11.Fengshuo Li.2023.Microbiome - based agents can optimize composting of agricultural wastes by modifying microbial communities.IF=11.889


12.Liu-ya Wang.2023.Effect of chitosan-ascorbic acid composite coating on postharvest quality of Custard apple (Annona squamosa).IF=4.885


13.Siqiang Wang.2023.Transcriptome analysis reveals key metabolic pathways and gene expression involving in cell wall polysaccharides-disassembling and postharvest fruit softening in custard apple (Annona squamosa L.)。IF=8.025


14.Wenqi Li.2023.Identification, pathogenic mechanism and control of Rhizopus oryzae causing postharvest fruit rot in pumpkin.IF=7

1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?

     答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。

          “24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。


2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?

     答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;

           微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。


3、问:分光法试剂盒与微板法试剂盒是否能通用?

     答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!