0512-66188056

产品中心

技术服务 试剂盒开发

总黄酮(TF)试剂盒

货号:G0118W
规格:96样
价格:710
说明书:点击下载
提示:官网说明书如与试剂盒内纸质版有出入,请以纸质版为准!

  • 产品简介
  • 已发文章
  • 产品问答

一、产品简介:

总黄酮,即黄酮类化合物;是植物重要的一类次生代谢产物,具有有较强的抗氧化活性,可捕捉活性氧自由基,降低氧化伤害,在果实中影响其色泽和风味;对植物的抗逆性和抗病虫害方面有重要作用。

本试剂盒采用 NaNO 2 -Al(NO 3 ) 3 -NaOH 显色法测定黄酮总含量,即在碱性亚硝酸盐溶液中,黄酮类化合物与铝离子形成在 510nm 处有特征吸收峰的红色络合物,测定反应产物在 510nm 处的吸光值,即可计算样品中总黄酮含量。

三、所需的仪器和用品

酶标仪、96孔板、粉碎仪、筛子、60%乙醇、离心机、蒸馏水。

  1. Feng Xiao.2022.Trade-off between shade tolerance and chemical resistance of invasive Phytolacca americana under different light levels compared with its native and exotic non-invasive congeners.IF=5.545


  2. Lei Liu.2020.Exogenous allantoin improves the salt tolerance of sugar beet by increasing putrescine metabolism and antioxidant activities.IF=3.72


  3. Yanhui Xiao.2021.Cinnamic acid treatment reduces the surface browning of fresh-cut taro.IF=3.463


  4. Yan Wang.2022.Beneficial effects of cadmium on plant defense of an invasive plant.IF=6.208


  5. Xie Wanfeng.2022.Expression of PmACRE1 in Arabidopsis thaliana enables host defence against Bursaphelenchus xylophilus infection.IF=5.26


  6. Fei Ren.2023.Deciphering Microbial Diversity and Functional Codes of Traditional Fermented Whole Grain Tianpei from Typical Regions of China.IF=5.123


  7. Liu, Yun-Shan.2023.Asymmetric inter-specific competition between invasive Phytolacca americana and its native congener.IF=1.99


  8. Bei Zhang.2023.Ectopic Expression of FvVND4c Promotes Secondary Cell Wall Thickening and Flavonoid Accumulation in Fragaria vesca.IF=6.208


  9. Yuanyuan Li.2023.Preparation of Thifluzamide Polylactic Acid Glycolic Acid Copolymer Microspheres and Its Effect on the Growth of Cucumber Seedlings.IF=5.6


  10. Ren Fei.2023.Core microbes closely related with the nutrients and flavor of sweet fermented oats (whole grain food) from China.IF=4.1


  11. Tang Danfeng.2023.Integrating LC-MS and HS-GC-MS for the metabolite characterization of the Chinese medicinal plant Platostoma palustre under different processing methods.IF=6.59


  12. Jiankang Xin.2023.A comprehensive view of metabolic responses to CYP98 perturbation in ancestral plants.IF=5.437


1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?

     答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。

          “24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。


2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?

     答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;

           微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。


3、问:分光法试剂盒与微板法试剂盒是否能通用?

     答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!