一、产品简介:
脂肪酶(EC 3.1.1.3)是一种特殊的酯键水解酶,能催化天然油脂水解,,在食品、医药、洗涤剂和皮革等许多工业领域中都有广泛的应用,
本试剂盒提供一种简单、快速的检测方法,以对硝基苯酚酯作为底物,脂肪酶水解底物产生具有颜色的对硝基苯酚,在405nm波长下测定其吸光值,即可得出脂肪酶活力。
二、所需的仪器和用品:
酶标仪、96孔板、研钵、低温离心机、可调式移液枪、冰和蒸馏水。
1. Yangyi Hao. 2021. Rumen Fermentation, Digestive Enzyme Activity, and Bacteria Composition between Pre-Weaning and Post-Weaning Dairy Calves. Animals. IF=2.75
2. Yangyi Hao. 2021. Effects of Age, Diet CP, NDF, EE, and Starch on the Rumen Bacteria Community and Function in Dairy Cattle. Microorganisms. IF=3.94
3.Silin Fan.2022.Melatonin Maintains Fruit Quality and Reduces Anthracnose in Postharvest Papaya via Enhancement of Antioxidants and Inhibition of Pathogen Development.IF=7.675
4.Silin Fan.2022.Melatonin Treatment Improves Postharvest Preservation and Resistance of Guava Fruit (Psidium guajava L.).IF=5.561
5.Yangyi Hao.2021.Effects of Age, Diet CP, NDF, EE, and Starch on the Rumen Bacteria Community and Function in Dairy Cattle.IF=4.128
6.Yangyi Hao.2021.Rumen Fermentation, Digestive Enzyme Activity, and Bacteria Composition between Pre-Weaning and Post-Weaning Dairy Calves.IF=2.752
7.Yujie Song.2022.Enzymes and microorganisms jointly promote the fermentation of rapeseed cake.IF=6.59
8.Jiaqi Wang.2023.l-Arginine treatment maintains postharvest quality in blueberry fruit by enhancing antioxidant capacity uring storage.IF=3.9
9.Juanjuan Chen.2023.Genome-Wide Detection of SPX Family and Profiling of CoSPX-MFS3 in Regulating Low-Phosphate Stress in Tea-Oil Camellia.IF=5.6
1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?
答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。
2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?
答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;
微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。
3、问:分光法试剂盒与微板法试剂盒是否能通用?
答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!