一、产品简介： 4-香豆酸：辅酶 A 连接酶（4CL ，EC 6.2.1.12）是木质素生物合成的关键酶之一，位于 苯丙酸途径与木质素特异合成途径的转折点上，主要催化肉桂酸生成相应的肉桂酸辅酶 A 酯。该酶主要存在于高等植物、酵母和菌类中，研究该酶可以探讨多种生物细胞发育过程 中木质素沉积的代谢机理，为减少水果石细胞含量而提高其品质提供依据。 4CL 催化 4-香豆酸和 CoA 生成 4-香豆酸 CoA，在 333nm 下测 4-香豆酸 CoA 生成速率， 即可反映 4CL 活性。

二、所需的仪器和用品： 酶标仪、96 孔板（UV 板）、低温台式离心机、可调式移液器、研钵、冰和蒸馏水。

1. Bin Cheng.2020. Effects of Multiple Planting Densities on Lignin Metabolism and Lodging Resistance of the Strip Intercropped Soybean Stem. Agronomy. IF=3.4

2. Bin Cheng. 2022. Shade-Tolerant Soybean Reduces Yield Loss by Regulating Its Canopy Structure and Stem Characteristics in the Maize–Soybean Strip Intercropping System. Frontiers in Plant Science. IF=5.75

3.Silin Fan.2022.Melatonin Maintains Fruit Quality and Reduces Anthracnose in Postharvest Papaya via Enhancement of Antioxidants and Inhibition of Pathogen Development.IF=7.675

4.Bin Cheng.2022.Shade-Tolerant Soybean Reduces Yield Loss by Regulating Its Canopy Structure and Stem Characteristics in the Maize–Soybean Strip Intercropping System..IF=6.627

5.Silin Fan.2022.Melatonin Treatment Improves Postharvest Preservation and Resistance of Guava Fruit (Psidium guajava L.).IF=5.561

6.Yanhui Xiao.2021.Cinnamic acid treatment reduces the surface browning of fresh-cut taro.IF=3.463

7.Jingyi Shao.2022.Combined effects of high temperature and waterlogging on yield and stem development of summer maize.IF=4.647

8.Lei Wang.2023.Dark septate endophyte Exophiala pisciphila promotes maize growth and alleviates cadmium toxicity.IF=6.064