一、产品简介:
还原型谷胱甘肽GSH是细胞内最主要的抗氧化巯基物质,在抗氧化、蛋白质巯基保护和氨基酸跨膜运输等中具有重要作用。还原型与氧化型比值(GSH/GSSG)是细胞氧化还原状态的主要动态指标。因此,测定细胞内GSH和GSSG含量以及GSH/GSSG比值,能够很好地反映细胞所处的氧化还原状态。
还原型谷胱甘肽GSH与DTNB与反应生成复合物,在412nm处有特征吸收峰;其吸光度与GSH含量成正比。
二、所需的仪器和用品:
可见分光光度计、1mL玻璃比色皿(光径1cm)、低温离心机、水浴锅、可调节移液器
1. Min Zhou. 2021. γ-Aminobutyric Acid (GABA) Priming Improves Seed Germination and Seedling Stress Tolerance Associated With Enhanced Antioxidant Metabolism, DREB Expression, and Dehydrin Accumulation in White Clover Under Water Stress. Frontiers in Plant Science. IF=5.57
2. Na Jiang.2022. Responses of antioxidant enzymes and key resistant substances in perennial ryegrass (Lolium perenne L.) to cadmium and arsenic stresses.BMC Plant Biology. IF=4.22
3. Cheng Wang. 2022. Hepatoprotective effect of phillygenin on carbon tetrachloride-induced liver fibrosis and its effects on short chain fatty acid and bile acid metabolism. Journal of Ethnopharmacology.
4. 刘梅,李祖然,张光群,王吉秀,祖艳群. 2020. 硫素对小花南芥体内含硫化合物与铅累积特征的影响.农业环境科学学报.
5. 牟凤利,杨京民,刘翠,刘才鑫,刘梅,陈建军,祖艳群,王吉秀.2021.野生小花南芥体内 AsA-GSH 循环对土壤 Cd、Pb 胁迫的响应.农业环境科学学报.
6. 孙晓莉.贾春燕.田寿乐.文燕.王金平.冉 昆.沈广宁. 2022. 外源甲基乙二醛对干旱胁迫下板栗幼苗的影响.应用生态学报.
7.Qi Chen.2021.Intervening oxidative stress integrated with an excellent biocompatibility of hemodialysis membrane fabricated by nucleobase-recognized co-immobilization strategy of tannic acid, looped PEtOx brush and heparin.IF=8.742
8.Yan Li.2022.Jasmonic acid alleviates cadmium toxicity through regulating the antioxidant response and enhancing the chelation of cadmium in rice (Oryza sativa L.).IF=8.071
9.Miaomiao Yao.2021.Exogenous glutathione alleviates chilling injury in postharvest bell pepper by modulating the ascorbate-glutathione (AsA-GSH) cycle.IF=7.514
10.Qi Chen.2020.Tannic acid and Poly(N-acryloyl morpholine) layer-by-layer built hemodialysis membrane surface for intervening oxidative stress integrated with high biocompatibility and dialysis performance.IF=.183
11.Ming Gao.2022.Sex-specific physiological and biochemical responses of Litsea cubeba under waterlogging stress.IF=6.028
12.Min Zhou.2021.γ-Aminobutyric Acid (GABA) Priming Improves Seed Germination and Seedling Stress Tolerance Associated With Enhanced Antioxidant Metabolism, DREB Expression, and Dehydrin Accumulation in White Clover Under Water Stress.IF=5.754
13.Yueying Sang.2022.Transcriptome sequencing reveals mechanism of improved antioxidant capacity and maintained postharvest quality of winter jujube during cold storage after salicylic acid treatment.IF=5.537
14.Jiang, Na.2022.Responses of antioxidant enzymes and key resistant substances in perennial ryegrass (Lolium perenne L.) to cadmium and arsenic stresses.IF=5.26
15.Ying Zhou.2022.Proteomic Investigation of Molecular Mechanisms in Response to PEG-Induced Drought Stress in Soybean Roots.IF=4.658
16.Cheng Wang.2022.Hepatoprotective effect of phillygenin on carbon tetrachloride-induced liver fibrosis and its effects on short chain fatty acid and bile acid metabolism.IF=4.36
17.Lei Liu.2020.Exogenous allantoin improves the salt tolerance of sugar beet by increasing putrescine metabolism and antioxidant activities.IF=3.72
18.Jian Zhao.2022.Peptide OM-LV20 promotes structural and functional recovery of spinal cord injury in rats.IF=3.575
19.Congcong Wang.2019.Characterization of the cellular effects and mechanism of arsenic trioxide-induced hepatotoxicity in broiler chickens.IF=3.067
20.Chuang Zhang.2021.Vitexin ameliorates glycochenodeoxycholate-induced hepatocyte injury through SIRT6 and JAK2/STAT3 pathways.IF=2.699
21.Chen, Siting.2022.Overexpression of the intertidal seagrass 14-3-3 gene ZjGRF1 enhances the tolerance of transgenic Arabidopsis to salt and osmotic stress.IF=2.496
22.Yan Li.2022.Melatonin alleviates arsenite toxicity by decreasing the arsenic accumulation in cell protoplasts and increasing the antioxidant capacity in rice.IF=8.943
23.Yajing Wang.2022.Gaseous ozone treatment prolongs the shelf-life of fresh-cut kiwifruit by maintaining its ascorbic acid content.IF=6.056
24.Ma, Wenxue.2022.The role of selenoprotein M in nickel-induced pyroptosis in mice spleen tissue via oxidative stress.IF=5.19
25.Qibin Wu.2023.Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance.IF=8.025
26.Shufeng Wang.2023.Differentially-expressed genes related to glutathione metabolism and heavy metal transport reveals an adaptive, genotype-specific mechanism to Hg2+ exposure in rice (Oryza sativa L.).IF=9.988
27.Erting Fu.2023.Chitosan Reduces Damages of Strawberry Seedlings under High-Temperature and High-Light Stress.IF=3.949
28.Kunyan Guo.2023.A MOF-based pH-responsive dual controlled-release system for herbicide pretilachlor and safener AD-67 delivery that enhances the herbicidal efficacy and reduces side effects.IF=9.473
29.Xingang Li.2023.GmGSTU23 Encoding a Tau Class Glutathione S-Transferase Protein Enhances the Salt Tolerance of Soybean (Glycine max L.).IF=6.208
30.Falin He.2023.Mechanistic insights into pyridine exposure induced toxicity in model Eisenia fetida species: Evidence from whole-animal, cellular, and molecular-based perspectives.IF=8.8
31.Rong Wu.2023.GsNAC2 gene enhances saline-alkali stress tolerance by promoting plant growth and regulating glutathione metabolism in Sorghum bicolor.IF=3
32.Baihui Jiang.Genome-wide identification of Glutathione peroxidase (GPX) family genes and silencing TaGPX3.2A reduced disease resistance in wheat.plant physiology and biochemistry.IF=6.5
33.Peng Mu.Genomic features of a plant growth-promoting endophytic Enterobacter cancerogenus JY65 dominant in microbiota of halophyte Suaeda salsa.plant and soil.IF=4.9
1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?
答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。
2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?
答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;
微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。
3、问:分光法试剂盒与微板法试剂盒是否能通用?
答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!