一、产品简介：
土壤蔗糖酶又叫土壤蔗糖转化酶，因其在酸性介质中活性最大，因此土壤中常测的蔗糖酶亦是酸性转化酶。其对增加土壤中易溶性营养物质起着重要的作用，与土壤中的有机质、氮、磷含量、微生物活动和土壤呼吸强度有关，一般情况下，土壤肥力越高，蔗糖酶活性越强，因此该酶也是评价土壤熟化程度和肥力水平的一个指标。本试剂盒采用 DNS 比色法，即土壤蔗糖酶催化蔗糖降解产生还原糖，进一步与 3,5－二硝基水杨酸反应，生成有色氨基化合物，在 540nm 有特征光吸收，在一定范围内 540nm光吸收增加速率与土壤蔗糖酶活性成正比。
二、所需的仪器和用品：
酶标仪（波长设置：540nm）、96孔酶标板、台式离心机、水浴锅/恒温培养箱、 可调式移液器、甲苯
1.Bin Zhou. 2020. Spatial distribution of phthalate esters and the associated response of enzyme activities and microbial community composition in typical plastic shed vegetable soils in China. Ecotoxicology and Environmental Safety. IF=6.3
2.J.Y. Zheng. 2022. Forty-year-old orchards promote carbon storage by changing aggregate-associated enzyme activities and microbial communities. Catena. IF=5.20
3.Yinjie Zhang. 2021. Long-term organic substitution management affects soil phosphorus speciation and reduces leaching in greenhouse vegetable production. Journal of Cleaner Production. IF=9.3
4.ZHANG Yin-Jie. 2021. Effects of a decade of organic fertilizer substitution on vegetable yield and soil phosphorus pools, phosphatase activities, and the microbial community in a greenhouse vegetable production system. Journal of Integrative Agriculture. IF=2.8
5.Zaid Khan.2022.How Biochar Affects Nitrogen Assimilation and Dynamics by Interacting Soil and Plant Enzymatic Activities: Quantitative Assessment of 2 Years Potted Study in a Rapeseed-Soil System..IF=.6.627
6.J.Y. Zheng.2022.Forty-year-old orchards promote carbon storage by changing aggregate-associated enzyme activities and microbial communities.IF=6.367
7.Kai Cai.2021.Pyrolysis-assisted transesterification for accurate quantification of phospholipid fatty acids: Application to microbial community analysis in 1000-years paddy soil chronosequence.IF=6.114
8.Ruiting Wang.2022.Metabolomics and Microbiomics Reveal Impacts of Rhizosphere Metabolites on Alfalfa Continuous Cropping.IF=6.064
9.Yan Wang.2022.Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng.IF=6.064
10.Bin Zhou.2020.Spatial distribution of phthalate esters and the associated response of enzyme activities and microbial community composition in typical plastic-shed vegetable soils in China.IF=4.872
11.Shifa Xiong.2023.Transcriptome and proteome analyses reveal a critical role of the sucrose anabolic pathway in regulating Quercus fabri Hance axillary bud outgrowth.IF=6.449
12.Can Wang.2023.Effects of dibutyl phthalate on microbial community and the carbon cycle in salinized soil.IF=11.072
13.Yunxin Shen.2023.Differential Responses of Bacterial and Fungal Communities to Siderophore Supplementation in Soil Affected by Tobacco Bacterial Wilt (Ralstonia solanacearum).IF=4.5
1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
