一、产品简介:
土壤中的脲酶主要来源于微生物和植物,它仅能水解土壤中的尿素,最终产物是氨和碳酸。土壤脲酶活性与土壤的微生物数量、有机物质含量、全氮和速效氮含量呈正相关。常用土壤脲酶活性表征土壤的氮素状况。本试剂盒采用靛酚蓝比色法:即脲酶水解尿素产生NH3-N,其在强碱性介质中与次氯酸盐和苯酚反应,生成水溶性染料靛酚蓝,该物质在578nm有最大光吸收,其深浅与溶液中的NH3-N含量呈正比,进而得出土壤脲酶活力大小。
二、所需的仪器和用品:
可见分光光度计(波长设置:578nm)、1mL 玻璃比色皿(光径 1cm)、离心机、水浴锅、可调式移液器、甲苯
1.Bin Zhou. 2020. Spatial distribution of phthalate esters and the associated response of enzyme activities and microbial community composition in typical plasticshed vegetable soils in China. Ecotoxicology and Environmental Safety. IF=6.3
2.J.Y. Zheng. 2022. Forty-year-old orchards promote carbon storage by changing aggregate-associated enzyme activities and microbial communities. Catena. IF=5.20
3.Yinjie Zhang. 2021. Long-term organic substitution management affects soil phosphorus speciation and reduces leaching in greenhouse vegetable production. Journal of Cleaner Production. IF=9.3
4.ZHANG Yin-Jie. 2021. Effects of a decade of organic fertilizer substitution on vegetable yield and soil phosphorus pools, phosphatase activities, and the microbial community in a greenhouse vegetable production system. Journal of Integrative Agriculture. IF=2.8
5.Zaid Khan.2022.How Biochar Affects Nitrogen Assimilation and Dynamics by Interacting Soil and Plant Enzymatic Activities: Quantitative Assessment of 2 Years Potted Study in a Rapeseed-Soil System..IF=.6.627
6.Kai Cai.2021.Pyrolysis-assisted transesterification for accurate quantification of phospholipid fatty acids: Application to microbial community analysis in 1000-years paddy soil chronosequence.IF=6.114
7.Ruiting Wang.2022.Metabolomics and Microbiomics Reveal Impacts of Rhizosphere Metabolites on Alfalfa Continuous Cropping.IF=6.064
8.Yan Wang.2022.Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng.IF=6.064
9.Bin Zhou.2020.Spatial distribution of phthalate esters and the associated response of enzyme activities and microbial community composition in typical plastic-shed vegetable soils in China.IF=4.872
10.Bin Zhou.2022.Effects of fertilizer application on phthalate ester pollution and the soil microbial community in plastic-shed soil on long-term fertilizer experiment.IF=8.943
11.Bei Zhang.2022.Characteristics of nitrogen distribution and its response to microecosystem changes in green infrastructure with different woody plants.IF=8.943
12.Ding Wang.2023.Maize-potato residue mixing in agricultural soils enhances residue decomposition and stable carbon content by modifying the potential keystone microbial taxa.IF=6.1
13.Yunxin Shen.2023.Differential Responses of Bacterial and Fungal Communities to Siderophore Supplementation in Soil Affected by Tobacco Bacterial Wilt (Ralstonia solanacearum).IF=4.5
14.Linxi Chang.2023.Effects of UV-B Radiation on the Chemical Composition of Azolla and Its Decomposition after Returning to the Field and Nitrogen Transformation in Soil.IF=3.7
15.Shaoguan Zhao.The impact of main Areca Catechu root exudates on soil microbial community structure and function in coffee plantation soils.Frontiers in Microbiology.IF=5.2
1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?
答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。
2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?
答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;
微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。
3、问:分光法试剂盒与微板法试剂盒是否能通用?
答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!