一、产品简介：
BCA蛋白含量试剂盒提供一种简单，快速，耐去污剂（最多5％）的检测蛋白质浓度的方法。由于蛋白质能将Cu2+还原成Cu+；BCA可与Cu+结合生成紫蓝色复合物，在562nm处有最大光吸光值，颜色的深浅与蛋白含量成正比，因此可根据吸光值测定蛋白质浓度。
二、所需的仪器和用品：
酶标仪、96孔板、台式离心机、恒温水浴锅、移液器和蒸馏水。
1.《Evaluating physiological changes of grass and semishrub species with seasonality for understanding the process of shrub encroachment in semiarid grasslands》.2021.《Functional Plant Biology》.IF=3.1
2.《Transcriptome and Co-expression Network Analyses Reveal Differential Gene Expression and Pathways in Response to Severe Drought Stress in Peanut (Arachis hypogaea L.)》.2021.《Frontiers in Genetics》.IF=4.6
3.《Resveratrol improves the iron defciency adaptation of Malus baccata seedlings by regulating iron absorption》.2021.《BMC Plant Biol》.IF=4.2
4.《Effects of root characteristics on panicle formation in japonica rice under low temperature water stress at the reproductive stage》.2021.《Field Crops Research》.IF=5.224
5.Daizong Cui.2020.The cytotoxicity of endogenous CdS and Cd2+ ions during CdS NPs biosynthesis.IF=9.038
6.Jiemeng Tao.2022.Metagenomic insight into the microbial degradation of organic compounds in fermented plant leaves.IF=8.431
7.Pengbai Li.2022.Geminivirus C4 proteins inhibit GA signaling via prevention of NbGAI degradation, to promote viral infection and symptom development in N. benthamiana.IF=6.823
8.Zaid Khan.2022.How Biochar Affects Nitrogen Assimilation and Dynamics by Interacting Soil and Plant Enzymatic Activities: Quantitative Assessment of 2 Years Potted Study in a Rapeseed-Soil System..IF=.6.627
9.Ci-mei Wang.2022.Physiological and transcription analyses reveal regulatory pathways of 6-benzylaminopurine delaying leaf senescence and maintaining quality in postharvest Chinese flowering cabbage.IF=6.475
10.Tingting Li.2021.Resveratrol Alleviates the KCl Salinity Stress of Malus hupehensis Rhed.IF=5.754
11.Ali Raza.2022.Mechanistic Insights Into Trehalose-Mediated Cold Stress Tolerance in Rapeseed ( Brassica napus L.) Seedlings.IF=5.754
12.Raza Ali.2021.Integrated Analysis of Metabolome and Transcriptome Reveals Insights for Cold Tolerance in Rapeseed (Brassica napus L.).IF=5.754
13.Yan Jia.2021.Effects of root characteristics on panicle formation in japonica rice under low temperature water stress at the reproductive stage.IF=5.224
14.Sun, Zhijuan.2022.Brassinolide alleviates Fe deficiency-induced stress by regulating the Fe absorption mechanism in Malus hupehensis Rehd.IF=4.964
15.Zaid Khan.2021.Compensation of high nitrogen toxicity and nitrogen deficiency with biochar amendment through enhancement of soil fertility and nitrogen use efficiency promoted rice growth and yield.IF=4.745
16.Zhao Nannan.2021.Transcriptome and Co-expression Network Analyses Reveal Differential Gene Expression and Pathways in Response to Severe Drought Stress in Peanut (Arachis hypogaea L.).IF=4.599
17.Zheng Wang.2022.Comparing Efficacy of Different Biostimulants for Hydroponically Grown Lettuce (Lactuca sativa L.).IF=3.949
18.Xiaodong Zheng.2020.Exogenous Strigolactones alleviate KCl stress by regulating photosynthesis, ROS migration and ion transport in Malus hupehensis Rehd.IF=3.72
19.Changchang Shao.2022.Physiological and Biochemical Dynamics of Pinus massoniana Lamb. Seedlings under Extreme Drought Stress and during Recovery.IF=3.282
20.Fei Ren.2021.Bacterial and fungal communities of traditional fermented Chinese soybean paste (Doujiang) and their properties.IF=2.863
21.Liu Yang.2020.Evaluating physiological changes of grass and semishrub species with seasonality for understanding the process of shrub encroachment in semiarid grasslands.IF=2.617
22.Zuoliang Sun.2022.Use of hemicellulose-derived xylose for environmentally sustainable starch production by mixotrophic duckweed.IF=6.813
23.Huiyuan Situ.2022.Effects of cold atmospheric plasma on endogenous enzyme activity and muscle protein oxidation in Trachinotus ovatus.IF=9.231
24.Xiao Yang.2022.Pre-harvest Nitrogen Limitation and Continuous Lighting Improve the Quality and Flavor of Lettuce (Lactuca sativa L.) under Hydroponic Conditions in Greenhouse.IF=5.895
25.Chongxi Liu.2023.Integrated Physiological, Transcriptomic, and Metabolomic Analysis Reveals the Mechanism of Guvermectin Promoting Seed Germination in Direct-Seeded Rice under Chilling Stress.IF=5.895
26.Ren Fei.2023.Core microbes closely related with the nutrients and flavor of sweet fermented oats (whole grain food) from China.IF=4.1
1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
