在碱性溶液中，凡分子中含二个或二个以上酰胺基(—CO-NH2)或与此相似基团的化合 物均与二价铜离子作用，络合物呈紫色，这一反应称双缩脲反应。蛋白质分子含有众多肽 键(—CONH—)，可发生双缩脲反应，且呈色强度在一定浓度范围内与蛋白质含量成正比， 经光谱扫描 540nm 为最大吸波长。

    酶标仪、96 孔板、台式离心机、恒温水浴锅（恒温培养箱）、移液器。

1.《Evaluating physiological changes of grass and semishrub species with seasonality for understanding the process of shrub encroachment in semiarid grasslands》.2021.《Functional Plant Biology》.IF=3.1

2.《Transcriptome and Co-expression Network Analyses Reveal Differential Gene Expression and Pathways in Response to Severe Drought Stress in Peanut (Arachis hypogaea L.)》.2021.《Frontiers in Genetics》.IF=4.6

3.《Resveratrol improves the iron defciency adaptation of Malus baccata seedlings by regulating iron absorption》.2021.《BMC Plant Biol》.IF=4.2

4.《Effects of root characteristics on panicle formation in japonica rice under low temperature water stress at the reproductive stage》.2021.《Field Crops Research》.IF=5.224