一、产品简介：
内切-β-1,4-葡聚糖酶（EC3.2.1.4）存在于细菌、真菌和动物体内，是纤维素酶系的组份之一，这类酶随机水解β-1,4-糖苷键，将无定形长链纤维素分子截短，将其分解成葡萄糖、纤维二糖、纤维三糖等各种类型的还原糖，在碱性条件下，产生的还原糖能与3,5-二硝基水杨酸反应生成棕红色物质，该物质在540nm下有最大吸收峰，即可得出内切-β-1,4-葡聚糖酶的酶活性大小。
二、所需的仪器和用品：
可见分光光度计、1mL玻璃比色皿（光径1cm）、水浴锅、离心机、可调式移液器、研钵、冰和蒸馏水。
1.《Inhibitory effects and mechanisms of vanillin on gray mold and black rot of cherry tomatoes》.2021.《Pesticide Biochemistry and Physiology》.IF=3.9
2.《Rumen Fermentation, Digestive Enzyme Activity, and Bacteria Composition between Pre-Weaning and Post-Weaning Dairy Calves》.2021.《Animals》.IF=2.75
3.《Effects of Age, Diet CP, NDF, EE, and Starch on the Rumen Bacteria Community and Function in Dairy Cattle》.2021.《Microorganisms》.IF=3.94
4.Chengze Yu.2021.Hydrothermal pretreatment contributes to accelerate maturity during the composting of lignocellulosic solid wastes.IF=9.624
5.Yi Gao.2022.Full-Scale of a Compost Process Using Swine Manure, Human Feces, and Rice Straw as Feedstock..IF=6.064
6.Dao-Jun Guo.2021.Differential Protein Expression Analysis of Two Sugarcane Varieties in Response to Diazotrophic Plant Growth-Promoting Endophyte Enterobacter roggenkampii ED5.IF=5.754
7.Yangyi Hao.2021.Rumen Fermentation, Digestive Enzyme Activity, and Bacteria Composition between Pre-Weaning and Post-Weaning Dairy Calves.IF=2.752
8.Fanlin Kong.2022.Preliminary Investigation of the Effects of Rosemary Extract Supplementation on Milk Production and Rumen Fermentation in High-Producing Dairy Cows.IF=7.675
9.Yujie Song.2022.Enzymes and microorganisms jointly promote the fermentation of rapeseed cake.IF=6.59
10.Yu-Xuan Wu.2022.Inhibitory effect and mechanism of action of juniper essential oil on gray mold in cherry tomatoes.IF=6.064
11.Fengshuo Li.2023.Microbiome - based agents can optimize composting of agricultural wastes by modifying microbial communities.IF=11.889
12.Liu-ya Wang.2023.Effect of chitosan-ascorbic acid composite coating on postharvest quality of Custard apple (Annona squamosa).IF=4.885
13.Siqiang Wang.2023.Transcriptome analysis reveals key metabolic pathways and gene expression involving in cell wall polysaccharides-disassembling and postharvest fruit softening in custard apple (Annona squamosa L.)。IF=8.025
14.Wenqi Li.2023.Identification, pathogenic mechanism and control of Rhizopus oryzae causing postharvest fruit rot in pumpkin.IF=7
1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
