一、产品简介:
淀粉酶包括α-淀粉酶(EC 3.2.1.1)和β-淀粉酶(EC 3.2.1.2)。淀粉酶催化淀粉水解生成还原糖,是生物体利用淀粉进行碳水化合物代谢的初级反应。本试剂盒采用70℃加热钝化β-淀粉酶来检测α-淀粉酶的活力。即α-淀粉酶催化淀粉水解生成的还原糖能使3,5-二硝基水杨酸生成棕红色得3-氨基-5-硝基水杨酸,在540 nm有吸收峰;通过测定540 nm吸光度增加速率,计算淀粉酶活性。
二、所需仪器和用品:
可见分光光度计、1mL玻璃比色皿(光径1cm)、恒温水浴锅、台式离心机、可调式移液器、研钵。
1. Dan Luo. 2020. Genome-Wide Analysis of the β-Amylase Gene Family in Brassica L. Crops and Expression Profiles of BnaBAM Genes in Response to Abiotic Stresses. Agronomy. IF=3.4
2. Yangyi Hao. 2021. Rumen Fermentation, Digestive Enzyme Activity, and Bacteria Composition between Pre-Weaning and Post-Weaning Dairy Calves. Animals. IF=2.75
3. Yangyi Hao. 2021. Effects of Age, Diet CP, NDF, EE, and Starch on the Rumen Bacteria Community and Function in Dairy Cattle. Microorganisms. IF=3.94
4.Liu, Rong.2021.Transcriptome and metabolome analyses revealing the potential mechanism of seed germination in Polygonatum cyrtonema.IF=4.38
5.Yangyi Hao.2021.Effects of Age, Diet CP, NDF, EE, and Starch on the Rumen Bacteria Community and Function in Dairy Cattle.IF=4.128
6.Yangyi Hao.2021.Rumen Fermentation, Digestive Enzyme Activity, and Bacteria Composition between Pre-Weaning and Post-Weaning Dairy Calves.IF=2.752
7.Yujie Song.2022.Enzymes and microorganisms jointly promote the fermentation of rapeseed cake.IF=6.59
8.Mengqi Zhang.2023.Effects of light on growth, feeding rate, digestion, and antioxidation in juvenile razor clams Sinonovacula constricta.IF=5.135
9.Wenjia Wang.2023.Transcriptomic and QTL Analysis of Seed Germination Vigor under Low Temperature in Weedy Rice WR04-6.IF=4.658
10.Pengfei Wan.2023.Enhanced degradation of extracellular polymeric substances by yeast in activated sludge to achieve sludge reduction.IF=11.889
11.Chongxi Liu.2023.Integrated Physiological, Transcriptomic, and Metabolomic Analysis Reveals the Mechanism of Guvermectin Promoting Seed Germination in Direct-Seeded Rice under Chilling Stress.IF=5.895
1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?
答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。
2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?
答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;
微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。
3、问:分光法试剂盒与微板法试剂盒是否能通用?
答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!