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木聚糖酶/半纤维素酶

货号:G0709W
规格:96样
价格:1020
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提示:官网说明书如与试剂盒内纸质版有出入,请以纸质版为准!

  • 产品简介
  • 已发文章
  • 产品问答

一、产品简介:

半纤维素酶主要检测木聚糖酶活力,可将木聚糖降解成低聚糖和木糖的一组酶的总称,广泛应用于酿造和饲料工业中。

半纤维素酶在酸性下将木聚糖降解成还原性寡糖和单糖,进一步与3,5-二硝基水杨酸发生显色反应,在540nm处有特征吸收峰,反应液颜色深浅与酶解产生的还原糖量成正比,通过测定反应液在540nm吸光值增加速率,即可计算该酶活力大小。

二、所需的仪器和用品:

酶标仪、96孔板、低温离心机、恒温水浴锅、可调式移液器、研钵、冰和蒸馏水。


1. Yangyi Hao. 2021. Rumen Fermentation, Digestive Enzyme Activity, and Bacteria Composition between Pre-Weaning and Post-Weaning Dairy Calves. Animals. IF=2.75

 

2. Yangyi Hao. 2021. Effects of Age, Diet CP, NDF, EE, and Starch on the Rumen Bacteria Community and Function in Dairy Cattle. Microorganisms. IF=3.94


3.Chengze Yu.2021.Hydrothermal pretreatment contributes to accelerate maturity during the composting of lignocellulosic solid wastes.IF=9.624


4.Yangyi Hao.2021.Effects of Age, Diet CP, NDF, EE, and Starch on the Rumen Bacteria Community and Function in Dairy Cattle.IF=4.128


5.Mingyang He.2021.Valeric acid suppresses cell wall polysaccharides disassembly to maintain fruit firmness of harvested ‘Waizuili’ plum (Prunus salicina Lindl).IF=3.463


6.Yangyi Hao.2021.Rumen Fermentation, Digestive Enzyme Activity, and Bacteria Composition between Pre-Weaning and Post-Weaning Dairy Calves.IF=2.752


7.Fanlin Kong.2022.Preliminary Investigation of the Effects of Rosemary Extract Supplementation on Milk Production and Rumen Fermentation in High-Producing Dairy Cows.IF=7.675


8.Yujie Song.2022.Enzymes and microorganisms jointly promote the fermentation of rapeseed cake.IF=6.59


9.Wenqi Li.2023.Identification, pathogenic mechanism and control of Rhizopus oryzae causing postharvest fruit rot in pumpkin.IF=7

1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?

     答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。

          “24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。


2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?

     答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;

           微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。


3、问:分光法试剂盒与微板法试剂盒是否能通用?

     答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!