内切-β-1,4-葡聚糖酶(EC3.2.1.4)存在于细菌、真菌和动物体内,是纤维素酶系的组份 之一,这类酶随机水解β-1,4-糖苷键,将无定形长链纤维素分子截短,将其分解成葡萄糖、 纤维二糖、纤维三糖等各种类型的还原糖,在碱性条件下,产生的还原糖能与3,5-二硝基 水杨酸反应生成棕红色物质,该物质在540nm下有最大吸收峰,即可得出内切-β-1,4-葡聚 糖酶的酶活性大小。
可见分光光度计、1mL 玻璃比色皿(光径 1cm)、水浴锅、离心机、可调式移液器、研钵。
1.《Inhibitory effects and mechanisms of vanillin on gray mold and black rot of cherry tomatoes》.2021.《Pesticide Biochemistry and Physiology》.IF=3.9
2.《Rumen Fermentation, Digestive Enzyme Activity, and Bacteria Composition between Pre-Weaning and Post-Weaning Dairy Calves》.2021.《Animals》.IF=2.75
3.《Effects of Age, Diet CP, NDF, EE, and Starch on the Rumen Bacteria Community and Function in Dairy Cattle》.2021.《Microorganisms》.IF=3.94