一、产品简介:
α-酮戊二酸脱氢酶(α-KGDH,EC 1.2.4.2)是三羧酸循环调控关键酶之一,广泛存在于动物、植物微生物和培养细胞中。可催化α-酮戊二酸、NAD+ 和辅酶A生成琥珀酰辅酶A、 二氧化碳和 NADH,通过检测NADH在340 nm的上升速率即可得出α-KGDH酶活性大小。
二、所需的仪器和用品:
紫外分光光度计、1mL石英比色皿(光径1cm)、水浴锅、台式离心机、可调式移液器、研钵、冰和蒸馏水。
Mingyang He.2022.Fluopyram inhibits tricarboxylic acid cycle but induces γ-aminobutyric acid-shunt pathway to prolong postharvest shelf life of litchi fruit.IF=6.751
Meng Tan.2022.Polyamines Metabolism Interacts with γ-Aminobutyric Acid, Proline and Nitrogen Metabolisms to Affect Drought Tolerance of Creeping Bentgrass.IF=6.208
Zhiling Tang.2022.Unraveling the antibacterial mechanism of 3-carene against Pseudomonas fragi by integrated proteomics and metabolomics analyses and its application in pork.IF=5.911
Yanhua Chen.2021.Rice spikelet formation inhibition caused by decreased sugar utilization under high temperature is associated with brassinolide decomposition.IF=5.545
Chen Yanhua.2023.Brassinosteroids Mediate Endogenous Phytohormone Metabolism to Alleviate High Temperature Injury at Panicle Initiation Stage in Rice.IF=4.412
Xue-Jing Huang.2023.Exogenous γ-aminobutyric acid (GABA) alleviates nitrogen deficiency by mediating nitrate uptake and assimilation in Andrographis paniculata seedlings.IF=5.437
Yunxia Qin.The role of citrate synthase HbCS4 in latex regeneration of Hevea brasiliensis (para rubber tree).industrial crops and products.IF=5.9
1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?
答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。
2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?
答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;
微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。
3、问:分光法试剂盒与微板法试剂盒是否能通用?
答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!