一、产品简介：
苯丙氨酸解氨酶（PAL，EC 4.3.1.24）是催化苯丙烷类代谢途径第一步反应的酶，也是这个途径的关键酶和限速酶，与一些重要的次生物质如木质素、异黄酮类植保素、黄酮类色素等合成密切相关，另外研究发现许多植物在遭受寒冷、伤害、紫外辐射时，植物的防卫体系特别是苯丙烷类代谢被激活，PAL活性迅速上升，因此PAL活性也可以作为植物抗逆境能力的一个生理指标。
本试剂盒根据苯丙氨酸解氨酶PAL催化L-苯丙氨酸裂解为反式肉桂酸和氨，反式肉桂酸在290nm处有最大吸收值，通过测定吸光值升高速率计算PAL活性。
二、所需的仪器和用品：
酶标仪、96孔板(UV板)、水浴锅、离心机、可调式移液器、研钵、冰和蒸馏水。
【注】：普通酶标板只能透过可见光，不能透过紫外光，检测波长小于340nm必须使用UV板
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1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
