一、产品简介:
4-香豆酸:辅酶A连接酶(4CL ,EC 6.2.1.12)是木质素生物合成的关键酶之一,位于苯丙酸途径与木质素特异合成途径的转折点上,主要催化肉桂酸生成相应的肉桂酸辅酶A酯。该酶主要存在于高等植物、酵母和菌类中,研究该酶可以探讨多种生物细胞发育过程中木质素沉积的代谢机理,为减少水果石细胞含量而提高其品质提供依据。
4CL催化4-香豆酸和CoA生成4-香豆酸CoA,在333nm下测4-香豆酸CoA生成速率,即可反映4CL活性。
二、所需的仪器和用品:
紫外分光光度计、1mL石英比色皿(光径1cm)、低温台式离心机、可调式移液器、研钵、冰和蒸馏水。
序号 | 论文标题 | 期刊 | IF | 原文链接 |
1 | Cinnamic acid treatment reduces the surface browning of fresh-cut taro | SCIENTIA HORTICULTURAE | 3.463 | https://www.sciencedirect.com/science/article/pii/S0304423821007202 |
2 | Melatonin Treatment Improves Postharvest Preservation and Resistance of Guava Fruit (Psidium guajava L.) | Foods | 5.561 | https://www.mdpi.com/2304-8158/11/3/262 |
3 | Shade-Tolerant Soybean Reduces Yield Loss by Regulating Its Canopy Structure and Stem Characteristics in the Maize–Soybean Strip Intercropping System | Frontiers in Plant Science | 6.627 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967133/ |
4 | Melatonin Maintains Fruit Quality and Reduces Anthracnose in Postharvest Papaya via Enhancement of Antioxidants and Inhibition of Pathogen Development | Antioxidants | 7.675 | https://www.mdpi.com/2076-3921/11/5/804 |
5 | Combined effects of high temperature and waterlogging on yield and stem development of summer maize | Crop Journal | 4.647 | https://www.sciencedirect.com/science/article/pii/S2214514122001957 |
6 | Dark septate endophyte Exophiala pisciphila promotes maize growth and alleviates cadmium toxicity | Frontiers in Microbiology | 6.064 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126344/ |
7 | Methyl jasmonate-loaded composite biofilm sustainably alleviates chilling lignification of loquat fruit during postharvest storage | FOOD CHEMISTRY | 8.8 | https://www.sciencedirect.com/science/article/pii/S0308814624002504 |
8 | Effects of UV-B Radiation Exposure on Transgenerational Plasticity in Grain Morphology and Proanthocyanidin Content in Yuanyang Red Rice | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 5.6 | https://www.mdpi.com/1422-0067/25/9/4766 |
1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?
答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。
2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?
答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;
微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。
3、问:分光法试剂盒与微板法试剂盒是否能通用?
答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!