一、产品简介:
丙二醛(MDA)是由于生物体官衰老或在逆境条件下受伤害,其组织或器官膜脂质发生过氧化反应而产生的。它的含量与生物体衰老及逆境伤害有密切关系。MDA 在高温、酸性条件下,与硫代巴比妥酸 (thiobarbituric acid,TBA)缩合,生成红色产物,在 532nm 有最大吸收峰,进行比色后可估测样品中过氧化脂质的含量;同时测定 600nm 下的吸光度,利用 532nm 与 600nm 下的吸光度的差值计算 MDA 的含量。
二、所需的仪器和用品:
酶标仪(波长设置:532nm与600nm)、96孔酶标板、水浴锅、台式离心机、可调式移液器、 研钵、
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10.Yi Zhang.2022.Analysis of Lhcb gene family in rapeseed (Brassica napus L.) identifies a novel member “BnLhcb3.4” modulating cold tolerance.IF=6.028
11.Hubiao Jiang.2021.Effect of the Nanoparticle Exposures on the Tomato Bacterial Wilt Disease Control by Modulating the Rhizosphere Bacterial Community.IF=5.924
12.Tingting Li.2021.Resveratrol Alleviates the KCl Salinity Stress of Malus hupehensis Rhed.IF=5.754
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15.Dao-Jun Guo.2021.Differential Protein Expression Analysis of Two Sugarcane Varieties in Response to Diazotrophic Plant Growth-Promoting Endophyte Enterobacter roggenkampii ED5.IF=5.754
16.Yang Wang.2021.Protective Effect of Lactobacillus plantarum P8 on Growth Performance, Intestinal Health, and Microbiota in Eimeria-Infected Broilers.IF=5.64
17.Fuqi Wang.2021.Isorhamnetin, the xanthine oxidase inhibitor from Sophora japonica, ameliorates uric acid levels and renal function in hyperuricemic mice.IF=5.396
18.Yan Jia.2021.Effects of root characteristics on panicle formation in japonica rice under low temperature water stress at the reproductive stage.IF=5.224
19.An, Peiqi.2022.Genetic transformation of LoHDZ2 and analysis of its function to enhance stress resistance in Larix olgensis.IF=4.996
20.Daowu Hu.2022.Identification and Characterization of the Growth-Regulating Factors-Interacting Factors in Cotton.IF=4.772
21.Shifa Xiong.2022.Effects of Drought Stress and Rehydration on Physiological and Biochemical Properties of Four Oak Species in China.IF=4.658
22.Ying Zhou.2022.Proteomic Investigation of Molecular Mechanisms in Response to PEG-Induced Drought Stress in Soybean Roots.IF=4.658
23.Zhao Nannan.2021.Transcriptome and Co-expression Network Analyses Reveal Differential Gene Expression and Pathways in Response to Severe Drought Stress in Peanut (Arachis hypogaea L.).IF=4.599
24.Liu, Xiaohui.2021.Study on browning mechanism of fresh-cut eggplant (Solanum melongena L.) based on metabolomics, enzymatic assays and gene expression.IF=4.38
25.Cheng Wang.2022.Hepatoprotective effect of phillygenin on carbon tetrachloride-induced liver fibrosis and its effects on short chain fatty acid and bile acid metabolism.IF=4.36
26.Xiaohui Liu.2022.Metabolomic Analysis, Combined with Enzymatic and Transcriptome Assays, to Reveal the Browning Resistance Mechanism of Fresh-Cut Eggplant.IF=4.35
27.Hong Zhu.2021.The sweetpotato β-amylase gene IbBAM1.1 enhances drought and salt stress resistance by regulating ROS homeostasis and osmotic balance.IF=4.27
28.Hong Zhu.2022.The Sweetpotato Voltage-Gated K+ Channel β Subunit, KIbB1, Positively Regulates Low-K+ and High-Salinity Tolerance by Maintaining Ion Homeostasis.IF=4.141
29.Xiaodong Zheng.2020.Exogenous Strigolactones alleviate KCl stress by regulating photosynthesis, ROS migration and ion transport in Malus hupehensis Rehd.IF=3.72
30.Jian Zhao.2022.Peptide OM-LV20 promotes structural and functional recovery of spinal cord injury in rats.IF=3.575
31.Lei Zhang.2021.Glutathione, carbohydrate and other metabolites of Larix olgensis A. Henry reponse to polyethylene glycol-simulated drought stress.IF=3.24
32.Chuang Zhang.2021.Vitexin ameliorates glycochenodeoxycholate-induced hepatocyte injury through SIRT6 and JAK2/STAT3 pathways.IF=2.699
33.Liu Yang.2020.Evaluating physiological changes of grass and semishrub species with seasonality for understanding the process of shrub encroachment in semiarid grasslands.IF=2.617
34.Jie Cui.2022.Transcriptome and Metabolome Analyses Revealed the Response Mechanism of Sugar Beet to Salt Stress of Different Durations.IF=6.208
35.Jiajia Wang.2022.LEAF TIP RUMPLED 1 Regulates Leaf Morphology and Salt Tolerance in Rice.IF=6.208
36.Yigong Zhang.2022.Structure, development, and the salt response of salt bladders in Chenopodium album L..IF=6.627
37.Yu-Xuan Wu.2022.Inhibitory effect and mechanism of action of juniper essential oil on gray mold in cherry tomatoes.IF=6.064
38.Chen, Siting.2022.Overexpression of Zostera japonica 14-3-3 gene ZjGRF1 enhances the resistance of transgenic Arabidopsis to copper stress.IF=2.742
39.Xiping Hou.2022.An insight into algicidal characteristics of Bacillus altitudinis G3 from dysfunctional photosystem and overproduction of reactive oxygen species.IF=8.943
40.Juan Wang.2022.Transcriptome and Metabolome Analyses Reveal Complex Molecular Mechanisms Involved in the Salt Tolerance of Rice Induced by Exogenous Allantoin.IF=7.675
41.Zhanyu Chen.2022.Molecular Characterization and Drought Resistance of GmNAC3 Transcription Factor in Glycine max (L.) Merr.IF=6.208
42.Mengyi Lin.2022.Naphthalimide-Based Fluorescent Probe for Profiling of Aldehydes during Oxidation of Unsaturated Lipids.IF=5.895
43.Shanshan Wang.2022.Development of Galloyl Antioxidant for Dispersed and Bulk Oils through Incorporation of Branched Phytol Chain.IF=4.927
44.Chen, Siting.2022.Overexpression of the intertidal seagrass J protein ZjDjB1 enhances tolerance to chilling injury.IF=2.496
45.Li Yang.2022.Quality Relationship between Smoked and Air-dried Bacon of Sichuan-Chongqing in China: Free Amino Acids, Volatile Compounds, and Microbial Diversity.IF=7.425
46.Huan Huang.2022.Unsaturated Fatty Acid Liposomes Selectively Regulate Glutathione Peroxidase 4 to Exacerbate Lipid Peroxidation as an Adaptable Liposome Platform for Anti-Tumor Therapy.IF=5.364
47.Qixian Wu.2022.Multiple metabolomics comparatively investigated the pulp breakdown of four dragon fruit cultivars during postharvest storage.IF=7.425
48.Sizhou Chen.2022.Hyperspectral machine-learning model for screening tea germplasm resources with drought tolerance.IF=6.627
49.Yun-Ze Chen.2022.Antifungal Activity of 6-Methylcoumarin against Valsa mali and Its Possible Mechanism of Action.IF=5.724
50.Jiajia Wang.2023.SEMI-ROLLED LEAF 10 stabilizes catalase isozyme B to regulate leaf morphology and thermotolerance in rice (Oryza sativa L.).IF=13.263
51.Jiao Du.2023.A prophage-encoded effector from “Candidatus Liberibacter asiaticus” targets ASCORBATE PEROXIDASE6 in citrus to facilitate bacterial infection.IF=5.52
52.Mengqi Zhang.2023.Effects of light on growth, feeding rate, digestion, and antioxidation in juvenile razor clams Sinonovacula constricta.IF=5.135
53.Na Li.2023.Phytic acid is a new substitutable plant-derived antifungal agent for the seedling blight of Pinus sylvestris var. mongolica caused by Fusarium oxysporum..IF=4.966
54.Li, Yanmei.2023.VaSUS2 confers cold tolerance in transgenic tomato and Arabidopsis by regulation of sucrose metabolism and ROS homeostasis.IF=4.964
55.Hang Yang.2023.Artemisia baimaensis allelopathy has a negative effect on the establishment of Elymus nutans artificial grassland in natural grassland.IF=2.734
56.Quanjun Zhan.2023.Effect of copper sulphate on Cryptocaryon irritans based on metabolome analysis.IF=2.58
57.Zhiyin Jiao.2023.Integration of transcriptome and metabolome analyses reveals sorghum roots responding to cadmium stress through regulation of the flavonoid biosynthesis pathway.IF=6.627
58.Xingang Li.2023.GmGSTU23 Encoding a Tau Class Glutathione S-Transferase Protein Enhances the Salt Tolerance of Soybean (Glycine max L.).IF=6.208
59.Dan He.2023.Antifungal activities of a novel triazole fungicide, mefentrifluconazole, against the major maize pathogen Fusarium verticillioides.IF=4.966
60.Liu B. S.2023.Effects of Light Intensity on Morphological Structure and Physiological Characteristics of Gleditsia sinensis Seedlings.IF=1.419
61.Qing-Qing Shen.2023.The SsWRKY1 transcription factor of Saccharum spontaneum enhances drought tolerance in transgenic Arabidopsis thaliana and interacts with 21 potential proteins to regulate drought tolerance in S. spontaneum.IF=5.437
62.Xiaomei Li.2023.Characterization of Chlorophyll Fluorescence and Antioxidant Defense Parameters of Two Gracilariopsis lemaneiformis Strains under Different Temperatures.IF=4.658
63.Chen Siting.2023.Overexpression of Zostera japonica J protein gene ZjDjB1 in Arabidopsis enhanced the tolerance to lead stress.IF=2.742
64.Song Jianfei.2023.MhCLC-c1, a Cl channel c homolog from Malus hupehensis, alleviates NaCl-induced cell death by inhibiting intracellular Cl– accumulation.IF=5.3
65Hong Zhu.2023.The Sweet Potato K+ Transporter IbHAK11 Regulates K+ Deficiency and High Salinity Stress Tolerance by Maintaining Positive Ion Homeostasis.IF=4.5
66.Jia-jun Li.2023.Genome-wide identification of the mango pathogenesis-related 1 (PR1) gene family and functional analysis of MiPR1A genes in transgenic Arabidopsis.IF=4.3
67.Hao Wu.2023.Disruption of LEAF LESION MIMIC 4 affects ABA synthesis and ROS accumulation in rice.IF=4.647
68.Zhijuan Sun.2023.Melatonin enhances KCl salinity tolerance by maintaining K+ homeostasis in Malus hupehensis.IF=13.8
69.Wenqi Li.2023.Identification, pathogenic mechanism and control of Rhizopus oryzae causing postharvest fruit rot in pumpkin.IF=7
70.Junliang Li.2023.Analysis of N6-methyladenosine reveals a new important mechanism regulating the salt tolerance of sugar beet (Beta vulgaris).IF=5.2
71.Hao Chen.2023.Enhancing the Adaptability of Tea Plants (Camellia sinensis L.) to High-Temperature Stress with Small Peptides and Biosurfactants.IF=4.5
72.Yingjie Yang.2023.Mineral and Metabolome Analyses Provide Insights into the Cork Spot Disorder on ‘Akizuki’ Pear Fruit.IF=3.1
73.Liu Jianguo.OsSTS, a Novel Allele of Mitogen-Activated Protein Kinase Kinase 4 (OsMKK4), Controls Grain Size and Salt Tolerance in Rice.Rice.IF=5.5
74.Lei Sun.Bacillus velezensis BVE7 as a promising agent for biocontrol of soybean root rot caused by Fusarium oxysporum.Frontiers in Microbiology.IF=5.2
75.Peng Mu.Genomic features of a plant growth-promoting endophytic Enterobacter cancerogenus JY65 dominant in microbiota of halophyte Suaeda salsa,plant and soil.IF=4.9
76.Ye Yuan.Promotional Properties of ACC Deaminase-Producing Bacterial Strain DY1-3 and Its Enhancement of Maize Resistance to Salt and Drought Stresses.Microorganisms.IF=4.5
1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?
答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。
2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?
答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;
微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。
3、问:分光法试剂盒与微板法试剂盒是否能通用?
答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!