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过氧化物酶(POD)试剂盒

货号:G0108W
规格:196样
价格:1100
说明书:点击下载
提示:官网说明书如与试剂盒内纸质版有出入,请以纸质版为准!

  • 产品简介
  • 产品问答
  • 相关论文


一、产品简介:

       过氧化物酶(POD,EC 1.11.1.7)广泛存在于动物、植物、微生物和培养细胞中, 是普遍存在的一种重要的氧化还原酶,其活性高低与抗性密切相关。在过氧化物酶催化 下,H2O2氧化愈创木酚生成红棕色产物,该产物在 470nm 处有最大光吸收,故可通过 测 470nm 下吸光值变化测定过氧化物酶活性。


二、需自备的仪器和用品:

         酶标仪、96 孔板、台式离心机、可调式移液器、研钵、冰和蒸馏水。


1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?

     答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。

          “24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。


2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?

     答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;

           微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。


3、问:分光法试剂盒与微板法试剂盒是否能通用?

     答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!



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