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超氧化物歧化酶(SOD)试剂盒-WST-8法

货号：G0102W
规格：196样
价格：1280
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  • 产品简介
  • 已发文章
  • 产品问答


一、产品简介：

     超氧化物歧化酶（SOD）（EC 1.15.1.1）在动植物、微生物和培养细胞体内广泛存在， 其具有抗衰老、提高机体对多种疾病的抵抗力，能增强机体对外界环境的适应力，是生物体内一种重要的抗氧化酶。目前有多种 SOD 活性测定法，其中 NBT(氮蓝四唑)法产生的甲臜染料水溶性差，易和被还原的黄嘌呤氧化酶相互作用，抑制百分率达不到 100%等，从而使检测的灵敏度和精确度受到影响；本试剂盒采用的是目前稳定性更好、灵敏度更高的 WST-8 法，WST-8可以和黄嘌呤氧化酶(Xanthine Oxidase, XO)催化产生的超氧化物阴离子(O2.- )反应产生水溶性的甲臜染料，后者在 450nm 处有最大吸收；SOD 可清除 O2.-.，从而抑制甲臜的形成；反应液颜色越深，说明 SOD 活性愈低，反之活性越高。


二、所需的仪器和用品：

      酶标仪(波长：450nm)、96 孔板、低温离心机、可调式移液器、研钵、冰和蒸馏水。



序号论文标题期刊IF原文链接
1H2S-Releasing Versatile Montmorillonite Nanoformulation Trilogically Renovates the Gut Microenvironment for Inflammatory Bowel Disease ModulationAdvanced Science15.1https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202308092
2Concurrence of microplastics and heat waves reduces rice yields and disturbs the agroecosystem nitrogen cycleJOURNAL OF HAZARDOUS MATERIALS14.224https://www.sciencedirect.com/science/article/pii/S0304389423006234
3Melatonin enhances KCl salinity tolerance by maintaining K+ homeostasis in Malus hupehensisPLANT BIOTECHNOLOGY JOURNAL13.8https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.14129
4Quorum sensing signal autoinducer-2 promotes hydrogen peroxide degradation in water by Gram-positive bacteriaJOURNAL OF HAZARDOUS MATERIALS13.6https://www.sciencedirect.com/science/article/pii/S0304389424001614
5Polystyrene microplastics promote intestinal colonization of Aeromonas veronii through inducing intestinal microbiota dysbiosisJOURNAL OF HAZARDOUS MATERIALS13.6https://www.sciencedirect.com/science/article/pii/S0304389424005557
6Effects of low concentration of gallic acid on the growth and microcystin production of Microcystis aeruginosaSCIENCE OF THE TOTAL ENVIRONMENT9.8https://www.sciencedirect.com/science/article/pii/S004896972308395X
7Exposure to atrazine stimulates progesterone secretion and induces oxidative stress, inflammation, and apoptosis in the ovary of pseudopregnant ratsCHEMOSPHERE8.8https://www.sciencedirect.com/science/article/pii/S0045653524007999
8Two transcription factors, the plant-specific B3 DNA-binding domain protein AcREM14 and the CCCH-zinc finger protein AcC3H1, confer resistance to Pseudomonas syringae pv. actinidiae in the Actinidia chinensis var. chinensis.Horticulture Research8.7https://academic.oup.com/hr/advance-article-abstract/doi/10.1093/hr/uhad242/7435258
9Integrative chemical, physiological, and metabolomics analyses reveal nanospecific phytotoxicity of metal nanoparticlesJOURNAL OF ENVIRONMENTAL MANAGEMENT8.7https://www.sciencedirect.com/science/article/pii/S0301479724003244
10Comparison of bio-beads combined with Pseudomonas edaphica and three phosphate materials for lead immobilization: Performance, mechanism and plant growthJOURNAL OF ENVIRONMENTAL MANAGEMENT8.7https://www.sciencedirect.com/science/article/pii/S0301479724007837
11Enhancing malting performance of harder barley varieties through ultrasound treatmentULTRASONICS SONOCHEMISTRY8.4https://www.sciencedirect.com/science/article/pii/S1350417724001081
12Synthesis of chitin nanocrystals supported Zn2+ with high activity against tobacco mosaic virusINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES8.2https://www.sciencedirect.com/science/article/pii/S0141813023030647
13Overexpression of MiSPL3a and MiSPL3b confers early flowering and stress tolerance in Arabidopsis thalianaINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES8.2https://www.sciencedirect.com/science/article/pii/S0141813024007165
14Coupling raw material cultivation with nano-hydroxyapatite application to utilize and remediate severely Cd-containing soilPROCESS SAFETY AND ENVIRONMENTAL PROTECTION7.8https://www.sciencedirect.com/science/article/pii/S0957582024001125
15Effects of Stocking Density on the Growth Performance, Physiological Parameters, Redox Status and Lipid Metabolism of Micropterus salmoides in Integrated Rice–Fish Farming SystemsAntioxidants7.675https://www.mdpi.com/2076-3921/11/7/1215
16Transcriptome and Metabolome Analyses Reveal Complex Molecular Mechanisms Involved in the Salt Tolerance of Rice Induced by Exogenous AllantoinAntioxidants7.675https://www.mdpi.com/1890910
17A bamboo bHLH transcription factor PeRHL4 has dual functions in enhancing drought and phosphorus starvation tolerancePLANT CELL AND ENVIRONMENT7.3https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.14920
18Cellobiose elicits immunity in lettuce conferring resistance againstJOURNAL OF EXPERIMENTAL BOTANY7.298https://academic.oup.com/jxb/advance-article-abstract/doi/10.1093/jxb/erac448/6831719
19Combined effects of S-metolachlor and benoxacor on embryo development in zebrafish (Danio rerio)ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY7.129https://www.sciencedirect.com/science/article/pii/S0147651322004055
20Melatonin Alleviates Antimony Toxicity by Regulating the Antioxidant Response and Reducing Antimony Accumulation in Oryza sativa L.Antioxidants7https://www.mdpi.com/2076-3921/12/11/1917
21ABC transporter SlABCG23 regulates chilling resistance of tomato fruit by affecting JA signaling pathwayPOSTHARVEST BIOLOGY AND TECHNOLOGY7https://www.sciencedirect.com/science/article/pii/S0925521423004234
22Flavonoid accumulation modulates the responses of cassava tuberous roots to postharvest physiological deteriorationPOSTHARVEST BIOLOGY AND TECHNOLOGY6.751https://www.sciencedirect.com/science/article/pii/S0925521423000157
23The regulatory pathway of transcription factor MYB36 from Trichoderma asperellum Tas653 resistant to poplar leaf blight pathogen Alternaria alternata Aal004MICROBIOLOGICAL RESEARCH6.7https://www.sciencedirect.com/science/article/pii/S0944501324000387
24Structure, development, and the salt response of salt bladders in Chenopodium album L.Frontiers in Plant Science6.627https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493005/
25Genome-wide analysis of the homeodomain-leucine zipper family in <i>Lotus japonicus</i> and the overexpression of <i>LjHDZ7</i> in Arabidopsis for salt tolerance.Frontiers in Plant Science6.627https://europepmc.org/articles/pmc9515785/bin/table_1.docx?javascript_support=no
26Integrating GWAS and transcriptomics to identify candidate genes conferring heat tolerance in riceFrontiers in Plant Science6.627https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868562/
27Integration of transcriptome and metabolome analyses reveals sorghum roots responding to cadmium stress through regulation of the flavonoid biosynthesis pathwayFrontiers in Plant Science6.627https://pubmed.ncbi.nlm.nih.gov/36909379/
28Candidate genes conferring ethylene-response in cultivated peanuts determined by BSA-seq and fine-mappingCrop Journal6.6https://www.sciencedirect.com/science/article/pii/S2214514124000576
29Developmental toxicity and transcriptome analysis of zebrafish (Danio rerio) embryos following exposure to chiral herbicide safener benoxacorSCIENCE OF THE TOTAL ENVIRONMENT6.551https://www.sciencedirect.com/science/article/pii/S0048969720368042
30Genome-wide identification of Glutathione peroxidase (GPX) family genes and silencing TaGPX3.2A reduced disease resistance in wheatPLANT PHYSIOLOGY AND BIOCHEMISTRY6.5https://www.sciencedirect.com/science/article/pii/S0981942823006502
31Metabolomics analysis of the nutraceutical diversity and physiological quality of Torreya yunnanensis seeds during cold storagePLANT PHYSIOLOGY AND BIOCHEMISTRY6.5https://www.sciencedirect.com/science/article/pii/S0981942823006940
32Physiological and Molecular Characteristics of Southern Leaf Blight Resistance in Sweet Corn Inbred LinesINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES6.208https://www.mdpi.com/1815860
33Physiological and Molecular Characteristics of Southern Leaf Blight Resistance in Sweet Corn Inbred LinesINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES6.208https://www.mdpi.com/1815860
34Molecular Characterization and Drought Resistance of GmNAC3 Transcription Factor in Glycine max (L.) Merr.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES6.208https://www.mdpi.com/1887308
35Cloning and Characterization of Two Novel PR4 Genes from Picea asperataINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES6.208https://www.mdpi.com/1974050
36GmGSTU23 Encoding a Tau Class Glutathione S-Transferase Protein Enhances the Salt Tolerance of Soybean (Glycine max L.)INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES6.208https://www.mdpi.com/1422-0067/24/6/5547
37Urea Cycle of Bacillus thuringiensis Affects Its Survival under UV StressJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY6.1https://pubs.acs.org/doi/abs/10.1021/acs.jafc.4c00167
38Inhibitory effect and mechanism of action of juniper essential oil on gray mold in cherry tomatoesFrontiers in Microbiology6.064https://pubmed.ncbi.nlm.nih.gov/36212845/
39Effect of yeast culture supplementation in sows during late gestation and lactation on growth performance, antioxidant properties, and intestinal microorganisms of offspring weaned pigletsFrontiers in Microbiology6.064https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9880171/
40Synergic mitigation of saline-alkaline stress in wheat plant by silicon and Enterobacter sp. FN0603Frontiers in Microbiology6.064https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885204/
41Sex-specific physiological and biochemical responses of Litsea cubeba under waterlogging stressENVIRONMENTAL AND EXPERIMENTAL BOTANY6.028https://www.sciencedirect.com/science/article/pii/S0098847222002404
42Effect of the Nanoparticle Exposures on the Tomato Bacterial Wilt Disease Control by Modulating the Rhizosphere Bacterial CommunityINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES5.924https://www.mdpi.com/1422-0067/23/1/414
43Mechanistic Insights into Stereospecific Antifungal Activity of Chiral Fungicide Prothioconazole against Fusarium oxysporum F. sp. cubenseINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES5.924https://www.mdpi.com/1422-0067/23/4/2352
44Protective Effect of Artocarpus heterophyllus Lam. (Jackfruit) Polysaccharides on Liver Injury Induced by Cyclophosphamide in MiceNutrients5.9https://www.mdpi.com/2072-6643/16/1/166
45Integrated Physiological, Transcriptomic, and Metabolomic Analysis Reveals the Mechanism of Guvermectin Promoting Seed Germination in Direct-Seeded Rice under Chilling StressJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY5.895https://pubs.acs.org/doi/abs/10.1021/acs.jafc.3c00559
46Resveratrol Alleviates the KCl Salinity Stress of Malus hupehensis RhedFrontiers in Plant Science5.754https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149799/
47Integrated Analysis of Metabolome and Transcriptome Reveals Insights for Cold Tolerance in Rapeseed (Brassica napus L.)Frontiers in Plant Science5.754https://www.frontiersin.org/articles/10.3389/fpls.2021.721681/full
48Differential Protein Expression Analysis of Two Sugarcane Varieties in Response to Diazotrophic Plant Growth-Promoting Endophyte Enterobacter roggenkampii ED5Frontiers in Plant Science5.754https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649694/
49Mechanistic Insights Into Trehalose-Mediated Cold Stress Tolerance in Rapeseed ( Brassica napus L.) SeedlingsFrontiers in Plant Science5.754https://pubmed.ncbi.nlm.nih.gov/35360297/
50Importance of abscisic acid and zeatin nucleosides for the nitrate-induced cadmium hyperaccumulation in Populus rootsENVIRONMENTAL AND EXPERIMENTAL BOTANY5.7https://www.sciencedirect.com/science/article/pii/S0098847224000728
51Protective Effect of Lactobacillus plantarum P8 on Growth Performance, Intestinal Health, and Microbiota in Eimeria-Infected BroilersFrontiers in Microbiology5.64https://www.frontiersin.org/articles/10.3389/fmicb.2021.705758/full
52Protective Effect of Lactobacillus plantarum P8 on Growth Performance, Intestinal Health, and Microbiota in Eimeria-Infected BroilersFrontiers in Microbiology5.64https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299996/
53A microencapsulation approach to design microbial seed coatings to boost wheat seed germination and seedling growth under salt stressFrontiers in Plant Science5.6https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10702945/
54Overexpression of a ‘Beta’ MYB Factor Gene, VhMYB15, Increases Salinity and Drought Tolerance in Arabidopsis thalianaINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES5.6https://www.mdpi.com/1422-0067/25/3/1534
55Polysaccharide from Artocarpus heterophyllus Lam. (Jackfruit) Pulp Ameliorates Dextran Sodium Sulfate-Induced Enteritis in RatsINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES5.6https://www.mdpi.com/1422-0067/25/3/1661
56Whole-Transcriptome Profiling and Identification of Cold Tolerance-Related ceRNA Networks in Japonica Rice VarietiesFrontiers in Plant Science5.6https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1260591/abstract
57Low-Toxicity Self-Photosensitized Biohybrid Systems for Enhanced Light-Driven H2 ProductionINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES5.6https://www.mdpi.com/1422-0067/25/6/3085
58Partial root-zone drying combined with nitrogen treatments mitigates drought responses in riceFrontiers in Plant Science5.6https://www.frontiersin.org/articles/10.3389/fpls.2024.1381491/full
59ZmNAC17 Regulates Mesocotyl Elongation by Mediating Auxin and ROS Biosynthetic Pathways in MaizeINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES5.6https://www.mdpi.com/1422-0067/25/9/4585
60OsSTS, a Novel Allele of Mitogen-Activated Protein Kinase Kinase 4 (OsMKK4), Controls Grain Size and Salt Tolerance in RiceRice5.5https://thericejournal.springeropen.com/articles/10.1186/s12284-023-00663-y
61The SsWRKY1 transcription factor of Saccharum spontaneum enhances drought tolerance in transgenic Arabidopsis thaliana and interacts with 21 potential proteins to regulate drought tolerance in S. spontaneumPLANT PHYSIOLOGY AND BIOCHEMISTRY5.437https://www.sciencedirect.com/science/article/pii/S0981942823002176
62Isorhamnetin, the xanthine oxidase inhibitor from Sophora japonica, ameliorates uric acid levels and renal function in hyperuricemic miceFood & Function5.396https://pubs.rsc.org/en/content/articlelanding/2021/fo/d1fo02719k
63Responses of antioxidant enzymes and key resistant substances in perennial ryegrass (Lolium perenne L.) to cadmium and arsenic stressesBMC PLANT BIOLOGY5.26https://link.springer.com/article/10.1186/s12870-022-03475-2
64Effects of root characteristics on panicle formation in japonica rice under low temperature water stress at the reproductive stageFIELD CROPS RESEARCH5.224https://www.sciencedirect.com/science/article/pii/S0378429021003415
65Bacillus velezensis BVE7 as a promising agent for biocontrol of soybean root rot caused by Fusarium oxysporumFrontiers in Microbiology5.2https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623355/
66Sulfur fertiliser enhancement of Erigeron breviscapus (Asteraceae) quality by improving plant physiological responses and reducing soil cadmium bioavailabilityENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH5.19https://link.springer.com/article/10.1007/s11356-022-20778-x
67Effects of light on growth, feeding rate, digestion, and antioxidation in juvenile razor clams Sinonovacula constrictaAQUACULTURE5.135https://www.sciencedirect.com/science/article/pii/S0044848623000790
68Role of reactive oxygen species (ROS) signaling pathway in the wound-induced spore formation of Pyropia yezoensisAlgal Research-Biomass Biofuels and Bioproducts5.1https://www.sciencedirect.com/science/article/pii/S2211926424000699
69Genetic transformation of LoHDZ2 and analysis of its function to enhance stress resistance in Larix olgensisScientific Reports4.996https://www.nature.com/articles/s41598-022-17191-2
70Differential effects of low and high temperature stress on pollen germination and tube length of mango (Mangifera indica L.) genotypesScientific Reports4.996https://www.nature.com/articles/s41598-023-27917-5
71Phytic acid is a new substitutable plant-derived antifungal agent for the seedling blight of Pinus sylvestris var. mongolica caused by Fusarium oxysporumPESTICIDE BIOCHEMISTRY AND PHYSIOLOGY4.966https://www.sciencedirect.com/science/article/pii/S0048357523000068
72VaSUS2 confers cold tolerance in transgenic tomato and Arabidopsis by regulation of sucrose metabolism and ROS homeostasisPLANT CELL REPORTS4.964https://link.springer.com/article/10.1007/s00299-022-02972-w
73Genomic features of a plant growth-promoting endophytic Enterobacter cancerogenus JY65 dominant in microbiota of halophyte Suaeda salsaPLANT AND SOIL4.9https://link.springer.com/article/10.1007/s11104-023-06360-5
74Assessing the toxicity of three “inert” herbicide safeners toward Danio rerio: Effects on embryos developmentECOTOXICOLOGY AND ENVIRONMENTAL SAFETY4.872https://www.sciencedirect.com/science/article/pii/S0147651320314135
75Effects of Dietary Supplementation of Lacticaseibacillus Chiayiensis AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao ChicksAntibiotics-Basel4.8https://www.mdpi.com/2079-6382/12/9/1356
76Appropriate Supply of Ammonia and Ammonium Nitrate Reduce Cadmium Content in Rice Seedlings by Inhibiting Cadmium Uptake and TransportRice Science4.8https://www.sciencedirect.com/science/article/pii/S1672630824000167
77Roles of Antioxidant Enzymes, Secondary Metabolites, and Lipids in Light Adaption of Tea-Oil Plant (Camellia oleifera Abel)JOURNAL OF PLANT GROWTH REGULATION4.8https://link.springer.com/article/10.1007/s00344-024-11256-4
78Trichoderma longibrachiatum Inoculation Improves Drought Resistance and Growth of Pinus massoniana Seedlings through Regulating Physiological Responses and Soil Microbial CommunityJournal of Fungi4.7https://www.mdpi.com/2309-608X/9/7/694
79Integrating transcriptome and physiological analyses to elucidate the molecular responses of sorghum to fluxofenim and metolachlor herbicidePESTICIDE BIOCHEMISTRY AND PHYSIOLOGY4.7https://www.sciencedirect.com/science/article/pii/S0048357523003577
80Effects of Drought Stress and Rehydration on Physiological and Biochemical Properties of Four Oak Species in ChinaPlants-Basel4.658https://www.mdpi.com/2223-7747/11/5/679
81Physiological and Transcriptome Analyses of CaCl2 Treatment to Alleviate Chilling Injury in PineapplePlants-Basel4.658https://www.mdpi.com/1796736
82Characterization of Chlorophyll Fluorescence and Antioxidant Defense Parameters of Two Gracilariopsis lemaneiformis Strains under Different TemperaturesPlants-Basel4.658https://www.mdpi.com/2223-7747/12/8/1670
83Disruption of LEAF LESION MIMIC 4 affects ABA synthesis and ROS accumulation in riceCrop Journal4.647https://www.sciencedirect.com/science/article/pii/S2214514123000491


1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？

     答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。

          “24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。


2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？

     答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;

           微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。


3、问：分光法试剂盒与微板法试剂盒是否能通用？

     答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！