一、产品简介：
超氧化物歧化酶（SOD）（EC 1.15.1.1）在动植物、微生物和培养细胞体内广泛存在， 其具有抗衰老、提高机体对多种疾病的抵抗力，能增强机体对外界环境的适应力，是生物体内一种重要的抗氧化酶。目前有多种 SOD 活性测定法，其中 NBT(氮蓝四唑)法产生的甲臜染料水溶性差，易和被还原的黄嘌呤氧化酶相互作用，抑制百分率达不到 100%等，从而使检测的灵敏度和精确度受到影响；本试剂盒采用的是目前稳定性更好、灵敏度更高的 WST-8 法，WST-8可以和黄嘌呤氧化酶(Xanthine Oxidase, XO)催化产生的超氧化物阴离子(O2.- )反应产生水溶性的甲臜染料，后者在 450nm 处有最大吸收；SOD 可清除 O2.-.，从而抑制甲臜的形成；反应液颜色越深，说明 SOD 活性愈低，反之活性越高。
二、所需的仪器和用品：
可见分光光度计(波长：450nm)、1mL 玻璃比色皿（光径 1cm）、低温离心机、可调式移液器、研钵
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39.Caiyun Xiong.2022.Physiological and Molecular Characteristics of Southern Leaf Blight Resistance in Sweet Corn Inbred Lines.IF=6.208
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1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
