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超氧化物歧化酶(SOD)试剂盒-WST-8法

货号:G0101W48
规格:48样
价格:400
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  • 产品简介
  • 已发文章
  • 产品问答

一、产品简介:                                           

超氧化物歧化酶(SOD)(EC 1.15.1.1)在动植物、微生物和培养细胞体内广泛存在,其具有抗衰老、提高机体对多种疾病的抵抗力,能增强机体对外界环境的适应力,是生物体内一种重要的抗氧化酶。

目前有多种SOD活性测定法,其中NBT(氮蓝四唑)法产生的甲臜染料水溶性差,易和被还原的黄嘌呤氧化酶相互作用,抑制百分率达不到100%等,从而使检测的灵敏度和精确度受到影响;本试剂盒采用的是目前稳定性更好、灵敏度更高的WST-8法,WST-8可以和黄嘌呤氧化酶(Xanthine Oxidase, XO)催化产生的超氧化物阴离子(O2.- )反应产生水溶性的甲臜染料,后者在450nm处有最大吸收;SOD可清除O2.-.,从而抑制甲臜的形成;反应液颜色越深,说明SOD活性愈低,反之活性越高。

 

二、所需的仪器和用品:

酶标仪、96孔板、低温离心机、可调式移液器、研钵、冰和蒸馏水。

 


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