一、产品简介：
谷胱甘肽过氧化物酶（GPx，EC 1.11.1.9）代表一种具有过氧化物酶活性的酶家族，在保护生物免受氧化损伤中起重要作用。以往以过氧化氢为底物进行检测，则会受到分解过氧化氢的过氧化氢酶(Catalase)的干扰，本试剂盒提供的有机过氧化物试剂(Cum-OOH)不会被过氧化氢酶分解，因而可以特异地检测出谷胱甘肽过氧化物酶的活力。 因此本试剂盒可以定量检测总谷胱甘肽过氧化物酶。
GSH和DTNB反应产生黄色物质，后者在412nm下有最大吸收峰，而GSH-Px催化有机过氧化物试剂(Cum-OOH)氧化GSH，使GSH量减少，GSH量减少越多，反应混合液黄色越浅，则GSH-Px活性越大；反之，黄色越深，GSH-Px活性越低。
二、所需的仪器和用品：
酶标仪、96孔板、低温离心机、移液器、研钵、冰和蒸馏水。
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